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Namespace Prefixes

Prefix	IRI
n7	http://purl.obolibrary.org/obo/
n3	http://model.geneontology.org/5323da1800000002/
dc	http://purl.org/dc/elements/1.1/
n8	http://purl.obolibrary.org/obo/#
rdfs	http://www.w3.org/2000/01/rdf-schema#
rdf	http://www.w3.org/1999/02/22-rdf-syntax-ns#
owl	http://www.w3.org/2002/07/owl#
xsdh	http://www.w3.org/2001/XMLSchema#
n5	http://purl.org/pav/
n2	http://geneontology.org/lego/

Statements

Subject Item

_:vb140787

rdf:type

owl:Axiom

rdfs:comment

"To test if our purified TFIIH and pol II were transcriptionally active, we first combined both epitope-tagged multiprotein complexes with bacterially expressed recombinant (r) human TFIIB, rTBP, rTFIIE, rTFIIF, a supercoiled DNA template (pG5MLT, which contains five Gal4-binding sites preceding the adenovirus major late core promoter elements), and a linearized DNA template (pMLΔ53, which contains the adenovirus major late core promoter elements without the Gal4-binding sites). As shown in Fig.5 A, both immunoaffinity-purified pol II complexes were equally active in transcribing the DNA templates, in conjunction with f:TFIIH, purified from the ammonium sulfate fraction, and other GTFs (comparelane 1 with lane 10)." PMID:9852112

dc:date

2018-05-01

dc:contributor

http://orcid.org/0000-0002-1706-4196

owl:annotatedProperty

n7:RO_0002333

owl:annotatedSource

n3:5323da180000008

owl:annotatedTarget

n8:5323da1800000002%2F5595c4cb00000117

n5:providedBy

http://www.wormbase.org

n2:evidence

n3:5ae3b0f600000054