"2017-08-25" . "https://syngo.vu.nl" . . "Slc8a2_MF_845" . "http://orcid.org/0000-0001-7351-8706" . "http://orcid.org/0000-0003-0938-8534" . "Rationale: The paper reports the cloning and functional characterization of NCX2 as a Ca2+/Na+ exchanger. The authors use 'inside out' patches from Xenopus oocytes expressing NCX2. \nThe patch pipette, facing the extracellular side, contains 8 mM Ca2+ and no Na+. \nThe external solution, facing the cytoplasmic side of the membrane patch, contains 100 mM cesium. Figure 5a shows that a current can be elicited when the cesium is replaced by Na+, in the presence of 1, or 3 microMol Ca2+ in the external solution. When no Ca2+ is present at the external solution, no current is elicited, indicating a requirement of Ca2+ at the cytoplasmic domain of the exchanger.\nFigure 5b shows that when the Ca2+ is removed from the external solution, the current decays.\nFigure 5d shows the IV curve for the exchanger.\n\nThe data presented indicates a function for NCX2 as a Na+/Ca2+ exchanger that is dependent on the ion gradients.. Experimental description: 'A rat genomic library (Stratagene) was then screened with the 0.6-kb probe. Four genomic clones were isolated and subcloned into pBluescript SK\u2019. Sequence analysis showed that one genomic clone, G23-2, overlapped with the 5\u2019 end of pRll and also had an apparent codon for the initiating methionine'.\n\n* 'We were initially unable to express NCX2 by injection of cRNA into Xenopus oocytes. We thus used the same strategy which improved expression of NCXl by replacing the 3\u2019-untranslated region of NCXl with that of the Na+-glucose cotransporter clone which includes a poly(A)+tail'." . . "http://orcid.org/0000-0002-0755-5899" . "production" . "http://orcid.org/0000-0003-3185-5709" .