<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/date>	"2017-11-23" .
<http://model.geneontology.org/SYNGO_799>	<http://geneontology.org/lego/modelstate>	"production" .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/contributor>	"http://orcid.org/0000-0003-3185-5709" .
<http://model.geneontology.org/SYNGO_799>	<http://www.w3.org/2002/07/owl#imports>	<http://purl.obolibrary.org/obo/go/extensions/go-lego.owl> .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/contributor>	"http://orcid.org/0000-0003-0938-8534" .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/title>	"P2rx3_CC_799" .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/contributor>	"http://orcid.org/0000-0002-0755-5899" .
<http://model.geneontology.org/SYNGO_799>	<http://www.w3.org/1999/02/22-rdf-syntax-ns#type>	<http://www.w3.org/2002/07/owl#Ontology> .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/pav/providedBy>	"https://syngo.vu.nl" .
<http://model.geneontology.org/SYNGO_799>	<http://purl.org/dc/elements/1.1/contributor>	"http://orcid.org/0000-0001-7351-8706" .
<http://model.geneontology.org/SYNGO_799>	<http://www.w3.org/2000/01/rdf-schema#comment>	"Rationale: Figure 1 shows enriched binding of P2X ligands to nerve terminals (synaptosomes). This is extended by subjecting the synaptosomes to differential detergent extraction at distinct pH (Figure 3 A).This highlights extraction of synaptosomes (syn) to generate biochemical fractions enriched for general presynaptic (pre), postsynaptic (post) of neuronal active zone (NAZ) components. P2X-3 is selectively extracted into the presynaptic membrane and further enriched in the NAZ. The figure (3B bottom of three panels) has selective staining with the same P2X-3 selective antibody on glass immobilized synaptosomes. (Antibodies quality controlled by Alamone).\n\n30/10/2017 Pim\n- Based on the known transmembrane topology of the protein, the \"integral component of ... membrane\" term was chosen.. Experimental description: Material and methods: Male Wistar rats  (8weeks)...." .