Rationale: Authors found nuclear and somatodendritic immunoreactivity of RNF10. At the synapses, RNF10 colocalized with PSD95 as observed with confocal microscopy and subcellular fractionation followed by western blotting revealed RNF10 in synaptic fractions and strongly present in the PSD fraction. Moreover, authors described a direct interaction between RNF10 and GluN2A-containing NMDA receptors. By using pull down assays, they described more in detail the binding region which is found in the N-terminal part of RNF10 and the GluN2A C-tail.
14/6/2017 Pim
- The "postsynaptic density" part is derived from the strong enrichment in the PSD fraction and the co-localization with PSD-95 (marker protein for PSD). The "extrinsic component of...." is derived from the colocalizaton with Glun2A (a transmembrane protein) and the ability to engage in direct PPI with Glun2A.. Experimental description: From M&M:
-Post-synaptic densities (PSDs) from rat hippocampus were used.
-Hippocampal neuronal primary cultures were prepared from embryonic day 18–19 (E18-E19) rat hippocampi.
-Co-immunoprecipitation assay was done using hippocampi from adult rats.
-shRNA target gene for RNF10 was accession number NM_001011904.1.
- For viral constructs, human flag-RNF10 sequence was used.
14/6/2017 Pim
- Species of GFP-Rnf10 protein used in Fig.1/2 is not mentioned in the paper. I disregard information on this protein since the data on the endogenous protein is sufficient for annotation.