Rationale: The dopamine transporter is an integral membrane protein of the postsynaptic membrane.
Pre-embedding immunogold and immunoperoxidase labelling demonstrates DAT-immunoreactivity predominantly along the plasma membrane in dendrites and dendritic spines (Fig. 1-3). The majority of the DAT-immunoreactivity was localized to tyrosine-hydroxylase positive neurons.
The DAT antiserum is a polyclonal antiserum directed against a synthetic peptide (aa42-59; LTNSTLINPPQTPVEAQE). The specificity of the antibody was demonstrated in a previous study (See reference 1). No KO-control.. Experimental description: Twelve adult male Sprague-Dawley rats (250-400 gm) were anesthetized...