Rationale: The paper quantitatively describes the distribution of GAT-1 and GAT-3 at the rat cortex using pre-embedding peroxidase labeling and postimbedding immunogold labeling.
Figure 3: electron microscopy images of peroxidase-based immunoreactivity in preembedded material of cortical layers 2-3. In figure 3d dendritic labeling is evident. Figure 3H quantifies the fraction of profiles where immunoreactivity was observed in axons or dendrites.
Figure 4h-n expands this analysis . In h-m example images of immunoreactivity in axon terminals, perisynaptic astrocytic processes and post-synaptic elements are shown. In figure 4n a quantification of fraction of profiles where immunoreactivity was observed in the above-mentioned cellular compartments, with a further division into symmetric and asymmetric synapses.
Taken together, the data supports a postsynaptic localization of GAT-3.. Experimental description: 'A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex'. 'Animals and tissue preparation Adult male albino rats (200–225 g; n = 7; Sprague–Dawley, Charles River, Milan, Italy) were used.'