Rationale: The paper examines the localization of kappa-type opioid receptor in dopaminergic terminals at the nucleus Accumbens using electron microscopic immunocytochemistry. Figure 1, 2 and 3 present immunoreactivities for kappa-type opioid receptors in presynaptic terminals, partially localizing to DAT immunoreactivities (quantified in figure 1). The immunostaining is found at the axonal plasma membrane and at the membrane of small SVs. 2/7/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.. Experimental description: 'Rats were anesthetized with 100 mg/kg i.p. sodium pentobarbital and perfused through the ascending aorta with 1) 10 ml of heparin (1,000 U/ml) in saline; 2) 50 ml of 3.75% acrolein (Polysciences, Warrington, PA)'