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Rationale: Pre-embedding immunoperoxidase and immunogold electron microscopy VAChT immunoreactivity in presynaptic terminals (Fig. 4c,d,e) and on synaptic vesicles (Fig. 4f,g,h), respectively. A rabbit polyclonal antibody directed against the C-terminus of VAChT was generated in the study. The specificity of the antibody was tested by Western Blotting (Fig. 1) and immunohistochemistry (Fig. 3 a,b). No KO-control.. Experimental description: Adult male Sprague-Dawley rats (n = 8) were given 300 IU of heparin i.p., deeply anesthetized with ...
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