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  • Rule: ActivityStreamsMapasEquivalentb3sb3sifpccisofacetshttp://demo.openlinksw.com/dataspacehttp://www.w3.org/2002/07/owl#ldpoplwebrel-me-authskos-transsql-vdb-data-lakeurn:ALKAdatareducedurn:analytics:acqof:ruleurn:clothing:rule:qaurn:det:rdf:labelurn:inference:oplfaq:ruleurn:inference:product:feature:inverse:ruleurn:ingleng:inf:demo:inf:ruleurn:kbpedia:actor:subclass:test:rulesurn:kbpedia:comedian:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:mime-artist:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:philosopher:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:stage-actor:owl:equivalent:class:subproperty:inference:rulesurn:kgdemo:1:ifp:ruleurn:kgdemo:1:ifp:rule2urn:lava:demo:ruleurn:owl:equivalent:class:inference:rulesurn:rdfs:subclass:subproperty:inference:rulesurn:rdfs:type:wikidata:ruleurn:recon:data:ifpurn:recon:data:ifp:ruleurn:recruitment:rule:built-in:ruleurn:rsm12e:translation:ruleurn:rxnorm:data:ruleurn:se:demo:inference:ruleurn:test:skos:inverse:ruleurn:wireless:plans:features:builtin:inference:rule:1virtrdf-eturtle-containersvirtrdf-hashtagvirtrdf-ifpvirtrdf-labelvirtrdf-meta-entity-classvirtrdf-urlNone
    
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  • "Same As": Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects (recommended) Apply to subjects, objects and predicates (not recommended on big datasets) Apply to predicates only (special use cases only)

Rationale: MacMARCKS was identified as an interaction partner of the presynaptic metabotropic glutamate receptor mGluR7 in a Y2H screen. Endogenous mGluR7 can be immunoprecipitated from cultured cerebellar granule cells and MacMARCKs can be co-immunoprecipitated (Fig. 5a). Overexpression of myc-tagged mGluR7 and GFP-tagged MacMARCKS in cultured cerebellar granule cells induces localization of both proteins to the plasma membrane of the soma and dendrites as assessed by ICC (Fig. 5f). Expression of a mGluR7 mutant devoid of the binding domain to MacMARCKS (mGluR7(ΔMacMARCKS) causes a retention of MacMARCKS in the cytoplasm of the cell (Fig. 5g). mGluR7 is involved in promoting G-protein-mediated inhibition of voltage-sensitive calcium channels (VSCCs). Co-expression of mGluR7A and MacMARCKS in cultured cerebellar granule cells strongly reduces tonic inhibition of VSCCs (Fig. 6b1) indicating an indirect role of MacMARCKS for the regulation of voltage-cated calcium channel activity. Antibody for WB was generated using purified GST-MacMARCKS fusion protein. Authors specify that the antibody was "not optimal for immunofluorescent labelling", therefore GFP-tagged MacMARCKS was overexpressed in cells for ICC. No KO-control. 25/8/2017 Pim - The MacMARCKS protein is inferred to exert the function at the presynapse based on (1) the mentioned presynaptic localization of the mGLUR7 and (2) the presence of MacMARCKs/MRP/MARCKSL1 in brain synapses (ticket #833). Experimental description: [...] a new-born rat brain cDNA library subcloned into the pAD vector were sequentially transformed into... To generate a MAcMARCKs expression plasmid, the entire coding sequence of MacMARCKs was amplified by PCR from the isolated pAD clone using...