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  • Rule: ActivityStreamsMapasEquivalentb3sb3sifpccisofacetshttp://demo.openlinksw.com/dataspacehttp://www.w3.org/2002/07/owl#ldpoplwebrel-me-authskos-transsql-vdb-data-lakeurn:ALKAdatareducedurn:analytics:acqof:ruleurn:clothing:rule:qaurn:det:rdf:labelurn:inference:oplfaq:ruleurn:inference:product:feature:inverse:ruleurn:ingleng:inf:demo:inf:ruleurn:kbpedia:actor:subclass:test:rulesurn:kbpedia:comedian:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:mime-artist:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:philosopher:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:stage-actor:owl:equivalent:class:subproperty:inference:rulesurn:kgdemo:1:ifp:ruleurn:kgdemo:1:ifp:rule2urn:lava:demo:ruleurn:owl:equivalent:class:inference:rulesurn:rdfs:subclass:subproperty:inference:rulesurn:rdfs:type:wikidata:ruleurn:recon:data:ifpurn:recon:data:ifp:ruleurn:recruitment:rule:built-in:ruleurn:rsm12e:translation:ruleurn:rxnorm:data:ruleurn:se:demo:inference:ruleurn:test:skos:inverse:ruleurn:wireless:plans:features:builtin:inference:rule:1virtrdf-eturtle-containersvirtrdf-hashtagvirtrdf-ifpvirtrdf-labelvirtrdf-meta-entity-classvirtrdf-urlNone
    
  • Inverse Functional Properties: Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects
  • "Same As": Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects (recommended) Apply to subjects, objects and predicates (not recommended on big datasets) Apply to predicates only (special use cases only)

Rationale: Rat hippocampal neurons expressing MARCKS RNAi constructs exhibit a greatly reduced spine density, spine length, and spine head width (Fig.1B). Efficiency of the KD was assessed by ICC (Fig. 1A). MARCKS influences number of spines and spine morphology in a phosphorylation-dependent manner. Overexpression of MARCKS constructs that mimic constitutively phosphorylated MARCKS (S4D-MARCKS and Dbl-MARCKS) and therefore have reduced membrane-association than wildtype-MARCKS in neurons causes a reduced spine protrusion length and head width (Fig.2). Overexpression of a MARCKS construct that mimic constitutively phosphorylated MARCKS (S4D-MARCKS) causes a reduction in total F-actin in dendrites in comparison to control neurons (Fig. 3A,B).. Experimental description: We used a RNA interference (RNAi) approach to address the role of endogenous MARCKS in the maintenance of dendritic morphology. We first examined the pattern of MARCKS expression in 3-week-old cultures of rat hippocampus. Full-length bovine eGFP-tagged wild-type-MARCKS, and four MARCKS mutants were a gift from P. Blackshear (Spizz and Blackshear, 2001)