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  • Rule: ActivityStreamsMapasEquivalentb3sb3sifpccisofacetshttp://demo.openlinksw.com/dataspacehttp://www.w3.org/2002/07/owl#ldpoplwebrel-me-authskos-transsql-vdb-data-lakeurn:ALKAdatareducedurn:analytics:acqof:ruleurn:clothing:rule:qaurn:det:rdf:labelurn:inference:oplfaq:ruleurn:inference:product:feature:inverse:ruleurn:ingleng:inf:demo:inf:ruleurn:kbpedia:actor:subclass:test:rulesurn:kbpedia:comedian:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:mime-artist:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:philosopher:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:stage-actor:owl:equivalent:class:subproperty:inference:rulesurn:kgdemo:1:ifp:ruleurn:kgdemo:1:ifp:rule2urn:lava:demo:ruleurn:owl:equivalent:class:inference:rulesurn:rdfs:subclass:subproperty:inference:rulesurn:rdfs:type:wikidata:ruleurn:recon:data:ifpurn:recon:data:ifp:ruleurn:recruitment:rule:built-in:ruleurn:rsm12e:translation:ruleurn:rxnorm:data:ruleurn:se:demo:inference:ruleurn:test:skos:inverse:ruleurn:wireless:plans:features:builtin:inference:rule:1virtrdf-eturtle-containersvirtrdf-hashtagvirtrdf-ifpvirtrdf-labelvirtrdf-meta-entity-classvirtrdf-urlNone
    
  • Inverse Functional Properties: Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects
  • "Same As": Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects (recommended) Apply to subjects, objects and predicates (not recommended on big datasets) Apply to predicates only (special use cases only)

Rationale: Whole cell patch recordings of P7-P10 calyxes at 22-24 oC were given a 20 ms depolarization from -80 mV to +10 mV. The initial rate of decay of the capacitance change was significantly slower (~33% of control) in the Actin, Gamma KO suggesting a slower rate of endocytosis following vesicle fusion (Fig 2. A-C). Similar results were observed when the stimulus was changed to a 20 action potential equivalent stimulus (Fig. 2 D-F), performed at 34-37 oC (Fig. 2 G) and in older mice (P13-P14) (Fig. 2 H). Hippocampal neurons were cultured from the Actg LoxP/LoxP mice transfected with Synaptophysin-pHluorin2X (SypH) with or without a Cre containing plasmid (Fig. 6 A and D). SypH can be used to study vesicle recycling as it is non-fluorescent in relatively low pH environment of vesicles but is fluorescent in the higher pH extracellular environment. Upon stimulation the decay of fluorescence was significantly slower than the control (Fig. 6 E-G). This fluorescence was quenchable by a low pH solution applied externally indicating it was not a deficit in vesicle reacidification, but instead suggesting that the vesicles had not been endocytosed (Fig. 6H). This effect was rescued by transfecting Actg in the KO cultures (Fig 7 A,B). Taken together this data suggests that Actin, Gamma is important for endocytosis. .. Experimental description: Krox20 Cre/+;Actg LoxP/LoxP mice were created, which knocked out Actin, Gamma in the Calyx of Held. 200 uM parasaggital brain stem slices were cut for capacitance recordings. Actg LoxP/LoxP were used as controls. Mouse hippocampal cultures were prepared using Actg LoxP/LoxP mice. Mice were transfected with a Cre-mCherry plasmid to create knockout. 8/6/2017 The species of the over-expression construct (Fig.7) is not relevant for species selection since serving as control for specificity of loss of native mouse protein.