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Rationale: In the paper the isolation of the EAAT5, an excitatory amino acid transporter is described. In figure 3 the uptake of 3H-L-glutamate and 3H-L-Aspertate are demonstrated in oocytes injected with the EAAT5 mRNA and voltage-clamped at −60 mV. In comparison to controls (uninjected oocytes), the uptake was typically 2- to 10-fold larger. The uptake is sodium- and voltage-dependent. These data indicate that the transporter functions as a glutamate transporter. Table 1 summarizes specificity and kinetic values of the transporter.. Experimental description: The isolation of an EAAT subtype from salamander retina whose sequence differed substantially from previously reported mammalian sequences lead us to isolate the human homolog EAAT5.

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  • Rationale: In the paper the isolation of the EAAT5, an excitatory amino acid transporter is described. In figure 3 the uptake of 3H-L-glutamate and 3H-L-Aspertate are demonstrated in oocytes injected with the EAAT5 mRNA and voltage-clamped at −60 mV. In comparison to controls (uninjected oocytes), the uptake was typically 2- to 10-fold larger. The uptake is sodium- and voltage-dependent. These data indicate that the transporter functions as a glutamate transporter. Table 1 summarizes specificity and kinetic values of the transporter.. Experimental description: The isolation of an EAAT subtype from salamander retina whose sequence differed substantially from previously reported mammalian sequences lead us to isolate the human homolog EAAT5.
Title
  • SYNGO_793
Date
  • 2017-08-28
Contributor
  • SynGO:SynGO-brose
http://purl.org/pav/providedBy
  • SynGO-VU
http://geneontology.org/lego/modelstate
  • production
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