Not logged in : Login
(Sponging disallowed)

About: Slc6a8_CC_774     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : owl:Ontology, within Data Space : demo.openlinksw.com associated with source document(s)

Rationale: The creatine transporter is a plasma membrane protein localized to the soma and neuronal processes of cultured rat hippocampal neurons (Fig. 3,4). Colabelling with MAP2 reveals a dendritic localization (Fig.3) and CRT co-localizes with the NR1 subunit of NMDA receptors (Fig.4) indicating a postsynaptic localization. CRT antibody was produced in rabbits and affinity-purified as described previously (Mak et al. 2009 - reference 2): "Briefly, antibodies were prepared by immunising rabbits with a C-terminal fragment of the bovine CRT, expressed as a fusion protein with glutathione-S-transferase (GST) from the parasitic helminth, Schistosoma japonicum. GST-reactive antibodies were removed on a column containing immobilised GST. Antibodies, specific for the C-terminal 21 amino acids of the CRT were affinity-purified on a column containing immobilised GST fusion protein (GST-CC2) and stored (0.5 mg/mL) in phosphate buffer (−80 °C). The affinity-purified CRT antibodies recognise a ∼70 kDa glycoprotein in membranes from human embryonic kidney (HEK) 293 cells, transiently or stably expressing the CRT and also showed strong staining of the plasma membrane of these cells by immunofluorescence microscopy." No KO control.. Experimental description: Briefly, E18 embryos from time-mated Wistar rats were removed from the uterus...

AttributesValues
type
imports
comment
  • Rationale: The creatine transporter is a plasma membrane protein localized to the soma and neuronal processes of cultured rat hippocampal neurons (Fig. 3,4). Colabelling with MAP2 reveals a dendritic localization (Fig.3) and CRT co-localizes with the NR1 subunit of NMDA receptors (Fig.4) indicating a postsynaptic localization. CRT antibody was produced in rabbits and affinity-purified as described previously (Mak et al. 2009 - reference 2): "Briefly, antibodies were prepared by immunising rabbits with a C-terminal fragment of the bovine CRT, expressed as a fusion protein with glutathione-S-transferase (GST) from the parasitic helminth, Schistosoma japonicum. GST-reactive antibodies were removed on a column containing immobilised GST. Antibodies, specific for the C-terminal 21 amino acids of the CRT were affinity-purified on a column containing immobilised GST fusion protein (GST-CC2) and stored (0.5 mg/mL) in phosphate buffer (−80 °C). The affinity-purified CRT antibodies recognise a ∼70 kDa glycoprotein in membranes from human embryonic kidney (HEK) 293 cells, transiently or stably expressing the CRT and also showed strong staining of the plasma membrane of these cells by immunofluorescence microscopy." No KO control.. Experimental description: Briefly, E18 embryos from time-mated Wistar rats were removed from the uterus...
Title
  • Slc6a8_CC_774
Date
  • 2017-08-21
Contributor
http://purl.org/pav/providedBy
http://geneontology.org/lego/modelstate
  • production
Faceted Search & Find service v1.17_git150 as of Jan 20 2025


Alternative Linked Data Documents: iSPARQL | ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 08.03.3332 as of Jan 29 2025, on Linux (x86_64-generic-linux-glibc25), Single-Server Edition (378 GB total memory, 17 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software