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  • Rule: ActivityStreamsMapasEquivalentb3sb3sifpccisofacetshttp://demo.openlinksw.com/dataspacehttp://www.w3.org/2002/07/owl#ldpoplwebrel-me-authskos-transsql-vdb-data-lakeurn:ALKAdatareducedurn:analytics:acqof:ruleurn:clothing:rule:qaurn:det:rdf:labelurn:inference:oplfaq:ruleurn:inference:product:feature:inverse:ruleurn:ingleng:inf:demo:inf:ruleurn:kbpedia:actor:subclass:test:rulesurn:kbpedia:comedian:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:mime-artist:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:philosopher:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:stage-actor:owl:equivalent:class:subproperty:inference:rulesurn:kgdemo:1:ifp:ruleurn:kgdemo:1:ifp:rule2urn:lava:demo:ruleurn:owl:equivalent:class:inference:rulesurn:rdfs:subclass:subproperty:inference:rulesurn:rdfs:type:wikidata:ruleurn:recon:data:ifpurn:recon:data:ifp:ruleurn:recruitment:rule:built-in:ruleurn:rsm12e:translation:ruleurn:rxnorm:data:ruleurn:se:demo:inference:ruleurn:test:skos:inverse:ruleurn:wireless:plans:features:builtin:inference:rule:1virtrdf-eturtle-containersvirtrdf-hashtagvirtrdf-ifpvirtrdf-labelvirtrdf-meta-entity-classvirtrdf-urlNone
    
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Rationale: The paper reports the cloning and functional characterization of NCX2 as a Ca2+/Na+ exchanger. The authors use 'inside out' patches from Xenopus oocytes expressing NCX2. The patch pipette, facing the extracellular side, contains 8 mM Ca2+ and no Na+. The external solution, facing the cytoplasmic side of the membrane patch, contains 100 mM cesium. Figure 5a shows that a current can be elicited when the cesium is replaced by Na+, in the presence of 1, or 3 microMol Ca2+ in the external solution. When no Ca2+ is present at the external solution, no current is elicited, indicating a requirement of Ca2+ at the cytoplasmic domain of the exchanger. Figure 5b shows that when the Ca2+ is removed from the external solution, the current decays. Figure 5d shows the IV curve for the exchanger. The data presented indicates a function for NCX2 as a Na+/Ca2+ exchanger that is dependent on the ion gradients.. Experimental description: 'A rat genomic library (Stratagene) was then screened with the 0.6-kb probe. Four genomic clones were isolated and subcloned into pBluescript SK’. Sequence analysis showed that one genomic clone, G23-2, overlapped with the 5’ end of pRll and also had an apparent codon for the initiating methionine'. * 'We were initially unable to express NCX2 by injection of cRNA into Xenopus oocytes. We thus used the same strategy which improved expression of NCXl by replacing the 3’-untranslated region of NCXl with that of the Na+-glucose cotransporter clone which includes a poly(A)+tail'.