• Facets (new session)
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• Metadata
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  • Rule: ActivityStreamsMapasEquivalentb3sb3sifpccisofacetshttp://demo.openlinksw.com/dataspacehttp://www.w3.org/2002/07/owl#ldpoplwebrel-me-authskos-transsql-vdb-data-lakeurn:ALKAdatareducedurn:analytics:acqof:ruleurn:clothing:rule:qaurn:det:rdf:labelurn:inference:oplfaq:ruleurn:inference:product:feature:inverse:ruleurn:ingleng:inf:demo:inf:ruleurn:kbpedia:actor:subclass:test:rulesurn:kbpedia:comedian:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:mime-artist:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:philosopher:owl:equivalent:class:subproperty:inference:rulesurn:kbpedia:stage-actor:owl:equivalent:class:subproperty:inference:rulesurn:kgdemo:1:ifp:ruleurn:kgdemo:1:ifp:rule2urn:lava:demo:ruleurn:owl:equivalent:class:inference:rulesurn:rdfs:subclass:subproperty:inference:rulesurn:rdfs:type:wikidata:ruleurn:recon:data:ifpurn:recon:data:ifp:ruleurn:recruitment:rule:built-in:ruleurn:rsm12e:translation:ruleurn:rxnorm:data:ruleurn:se:demo:inference:ruleurn:test:skos:inverse:ruleurn:wireless:plans:features:builtin:inference:rule:1virtrdf-eturtle-containersvirtrdf-hashtagvirtrdf-ifpvirtrdf-labelvirtrdf-meta-entity-classvirtrdf-urlNone
    
  • Inverse Functional Properties: Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects
  • "Same As": Disabled (fastest) Apply to subjects only Apply to objects only Apply to both subjects and objects (recommended) Apply to subjects, objects and predicates (not recommended on big datasets) Apply to predicates only (special use cases only)

Rationale: The proline transporter (PROT) is an integral membrane protein expressed in a subset of excitatory (glutamatergic, asymmetric) synapses in different brain regions. Pre- and postembedding immuno-EM reveals a localization of PROT predominately to synaptic vesicles in a subset of glutamatergic terminals, but also to the plasma membrane (Fig. 5B). Antibodies: Polyclonal rabbit antibody A2 was produced and affinity-purified by Research Genetics (Huntsville, AL) against residues 2–18 from the predicted N terminus of rat PROT (KKLQEAHLRKPVTPDLL). The affinity-purified polyclonal anti- body directed against the C terminus of rat PROT, antibody C597, has been described previously (Velaz-Faircloth et al., 1995). Both antibodies were tested for its specificity by biochemistry using stably PROT expressing HEK cells and transiently expressing HeLa cells (Fig.1). C597 antibody, preabsorbed with the C-terminal antigenic peptide, did not produce any immunoreactivity by immunohistochemistry (Fig.2D).. Experimental description: For electron microscopy, four adult (250–350 gm) male Sprague Daw- ley rats (Hilltop Lab) were anesthetized... Subcellular fractionation: Rat brain synaptic vesicles were purified from isolated nerve terminals...